| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2011-05-24 |
| タイトル |
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タイトル |
Genotyping of Polymorphisms in Alcohol and Aldehyde Dehydrogenase Genes by Direct Application of PCR-RFLP on Dried Blood without DNA Extraction |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| ID登録 |
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|
ID登録 |
10.14993/00000799 |
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ID登録タイプ |
JaLC |
| 著者 |
木下, 健司
横山, 顕
村田, 成範
小泉(岩尾), 恭子
林田, 真梨子
Iwao-Koizumi, Kyoko
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
We have developed a simple, labor-saving, inexpensive, and rapid single nucleotide polymorphism (SNP) genotyping method that works directly on whole human blood. This single-tube genotyping method was used to successfully and reliably genotype ADH1B and ALDH2 polymorphisms without DNA isolation using a 1.2-mm disc of dried blood and the KOD FX PCR enzyme kit. SNP genotyping was performed by a polymerase chain reaction?restriction fragment length polymorphism (PCR-RFLP) method. In addition to the labor and expense advantages, the possibility of sample contamination was considerably decreased, since the DNA extraction step was eliminated. In the post-genome era, a simple and inexpensive method for diagnostic analysis is in high demand, and this method will be very useful for genetic diagnoses in biological and medical laboratories. |
|
言語 |
en |
| 書誌情報 |
en : Analytical Sciences
巻 26,
号 4,
p. 503-505,
発行日 2010-04-01
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| 出版者 |
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出版者 |
Japan Society for Analytical Chemistry |
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言語 |
en |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0910-6340 |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA10500785 |
09106340_26(4)_503.pdf (580.4 kB)
