{"created":"2023-05-15T12:06:14.523016+00:00","id":805,"links":{},"metadata":{"_buckets":{"deposit":"96ed4421-7316-4b4a-8b50-3a231d65df04"},"_deposit":{"created_by":16,"id":"805","owners":[16],"pid":{"revision_id":0,"type":"depid","value":"805"},"status":"published"},"_oai":{"id":"oai:mukogawa.repo.nii.ac.jp:00000805","sets":["205"]},"author_link":[],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-04-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"505","bibliographicPageStart":"503","bibliographicVolumeNumber":"26","bibliographic_titles":[{"bibliographic_title":"Analytical Sciences","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We have developed a simple, labor-saving, inexpensive, and rapid single nucleotide polymorphism (SNP) genotyping method that works directly on whole human blood. This single-tube genotyping method was used to successfully and reliably genotype ADH1B and ALDH2 polymorphisms without DNA isolation using a 1.2-mm disc of dried blood and the KOD FX PCR enzyme kit. SNP genotyping was performed by a polymerase chain reaction?restriction fragment length polymorphism (PCR-RFLP) method. In addition to the labor and expense advantages, the possibility of sample contamination was considerably decreased, since the DNA extraction step was eliminated. In the post-genome era, a simple and inexpensive method for diagnostic analysis is in high demand, and this method will be very useful for genetic diagnoses in biological and medical laboratories.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.14993/00000799","subitem_identifier_reg_type":"JaLC"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japan Society for Analytical Chemistry","subitem_publisher_language":"en"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA10500785","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0910-6340","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"木下, 健司","creatorNameLang":"ja"},{"creatorName":"キノシタ, ケンジ","creatorNameLang":"ja-Kana"},{"creatorName":"KINOSHITA, Kenji","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"横山, 顕","creatorNameLang":"ja"},{"creatorName":"Yokoyama, Akira","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"村田, 成範","creatorNameLang":"ja"},{"creatorName":"ムラタ, シゲノリ","creatorNameLang":"ja-Kana"},{"creatorName":"MURATA, Shigenori","creatorNameLang":"en"}],"familyNames":[{"familyName":"村田","familyNameLang":"ja"},{"familyName":"ムラタ","familyNameLang":"ja-Kana"},{"familyName":"MURATA","familyNameLang":"en"}],"givenNames":[{"givenName":"成範","givenNameLang":"ja"},{"givenName":"シゲノリ","givenNameLang":"ja-Kana"},{"givenName":"Shigenori","givenNameLang":"en"}]},{"creatorNames":[{"creatorName":"小泉(岩尾), 恭子","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"林田, 真梨子","creatorNameLang":"ja"},{"creatorName":"ハヤシダ, マリコ","creatorNameLang":"ja-Kana"},{"creatorName":"HAYASHIDA, Mariko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Iwao-Koizumi, Kyoko","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-09-20"}],"displaytype":"detail","filename":"09106340_26(4)_503.pdf","filesize":[{"value":"580.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"09106340_26(4)_503.pdf","url":"https://mukogawa.repo.nii.ac.jp/record/805/files/09106340_26(4)_503.pdf"},"version_id":"0a4e5b10-a55d-42e1-a647-cc4dfd5a072b"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Genotyping of Polymorphisms in Alcohol and Aldehyde Dehydrogenase Genes by Direct Application of PCR-RFLP on Dried Blood without DNA Extraction","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Genotyping of Polymorphisms in Alcohol and Aldehyde Dehydrogenase Genes by Direct Application of PCR-RFLP on Dried Blood without DNA Extraction","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"16","path":["205"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2011-05-24"},"publish_date":"2011-05-24","publish_status":"0","recid":"805","relation_version_is_last":true,"title":["Genotyping of Polymorphisms in Alcohol and Aldehyde Dehydrogenase Genes by Direct Application of PCR-RFLP on Dried Blood without DNA Extraction"],"weko_creator_id":"16","weko_shared_id":-1},"updated":"2024-04-26T07:30:53.468531+00:00"}