| アイテムタイプ |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2009-07-01 |
| タイトル |
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|
タイトル |
An Improved Convenient Molecular Weight-determination Method for Active Stainable-Enzyme after SDS Electrophoresis |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| ID登録 |
|
|
ID登録 |
10.14993/00000364 |
|
ID登録タイプ |
JaLC |
| 著者 |
岡村, 徳光
堀江, 登
大杉, 匡弘
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
An improved method to determine the molecular weight of alcohol dehydrogenase after sodium dodecylsulfate polyacrylamide gel electrophoresis(SDS-PAGE) has been developed. This method was based on the finding that on a gel, which was washed with a buffer to remove SDS after SDS-PAGE, stained with an enzymatic activity staining mixture and then stained with coomassie blue, there appeared two active stained bands with apparent molecular weights of 148,000(tetramer)and 35,000(monomer)on the SDS-PAGE gel. The method developed here may be applicable to a wide range of active stainable-enzymes as a rapid and simple molecular weight determination method after SDS-PAGE. |
| 書誌情報 |
武庫川女子大学紀要. 自然科学編
巻 47,
p. 35-37,
発行日 2000-03-31
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| 出版者 |
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|
出版者 |
武庫川女子大学 |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0916-3123 |
| 書誌レコードID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN10187277 |
P035-037.pdf (242.9 kB)
