{"created":"2023-05-15T12:05:54.264302+00:00","id":370,"links":{},"metadata":{"_buckets":{"deposit":"14d6a572-4b0b-4f41-8a9f-ff9d22749ac2"},"_deposit":{"created_by":3,"id":"370","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"370"},"status":"published"},"_oai":{"id":"oai:mukogawa.repo.nii.ac.jp:00000370","sets":["267:271"]},"author_link":["1301","6149","6145"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2000-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"37","bibliographicPageStart":"35","bibliographicVolumeNumber":"47","bibliographic_titles":[{"bibliographic_title":"武庫川女子大学紀要. 自然科学編"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"An improved method to determine the molecular weight of alcohol dehydrogenase after sodium dodecylsulfate polyacrylamide gel electrophoresis(SDS-PAGE) has been developed. This method was based on the finding that on a gel, which was washed with a buffer to remove SDS after SDS-PAGE, stained with an enzymatic activity staining mixture and then stained with coomassie blue, there appeared two active stained bands with apparent molecular weights of 148,000(tetramer)and 35,000(monomer)on the SDS-PAGE gel. The method developed here may be applicable to a wide range of active stainable-enzymes as a rapid and simple molecular weight determination method after SDS-PAGE.","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.14993/00000364","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"武庫川女子大学"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10187277","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0916-3123","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"岡村, 徳光","creatorNameLang":"ja"},{"creatorName":"オカムラ, トクミツ","creatorNameLang":"ja-Kana"},{"creatorName":"OKAMURA, Tokumitsu","creatorNameLang":"en"}],"familyNames":[{"familyName":"岡村","familyNameLang":"ja"},{"familyName":"オカムラ","familyNameLang":"ja-Kana"},{"familyName":"OKAMURA","familyNameLang":"en"}],"givenNames":[{"givenName":"徳光","givenNameLang":"ja"},{"givenName":"トクミツ","givenNameLang":"ja-Kana"},{"givenName":"Tokumitsu","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6145","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"堀江, 登"},{"creatorName":"ホリエ, ノボル","creatorNameLang":"ja-Kana"},{"creatorName":"HORIE, Noboru","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1301","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大杉, 匡弘"},{"creatorName":"オオスギ, マサヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"OHSUGI, Masahiro ","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6149","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-09-25"}],"displaytype":"detail","filename":"P035-037.pdf","filesize":[{"value":"242.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"P035-037.pdf","url":"https://mukogawa.repo.nii.ac.jp/record/370/files/P035-037.pdf"},"version_id":"c3183f7c-c3a4-4fc7-909a-64a00b9f8716"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"An Improved Convenient Molecular Weight-determination Method for Active Stainable-Enzyme after SDS Electrophoresis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"An Improved Convenient Molecular Weight-determination Method for Active Stainable-Enzyme after SDS Electrophoresis","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"3","path":["271"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-07-01"},"publish_date":"2009-07-01","publish_status":"0","recid":"370","relation_version_is_last":true,"title":["An Improved Convenient Molecular Weight-determination Method for Active Stainable-Enzyme after SDS Electrophoresis"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2024-08-29T03:01:44.351862+00:00"}