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基質を含むポリアクリルアミド・ゲル電気泳動法によるヒト血漿中の中性セリンプロテイナーゼの検出の試み
https://doi.org/10.14993/00000332
https://doi.org/10.14993/0000033279b8c76a-c03e-435b-8e03-29ffb6afbae9
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
P107-111.pdf (645.1 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2009-06-11 | |||||||||||
| タイトル | ||||||||||||
| タイトル | 基質を含むポリアクリルアミド・ゲル電気泳動法によるヒト血漿中の中性セリンプロテイナーゼの検出の試み | |||||||||||
| タイトル | ||||||||||||
| タイトル | Studies on detection of neutral serine proteinase in human plasma by electrophoresis using substrate-containing polyacrylamide gel | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | jpn | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||
| ID登録 | ||||||||||||
| ID登録 | 10.14993/00000332 | |||||||||||
| ID登録タイプ | JaLC | |||||||||||
| 著者 |
堀江, 登
× 堀江, 登
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | In order to examine the existence of proteinase in the human plasma, the electrophoretic method by using polyacrylamide-gel containing SDS and substrate was adopted in this study. The proteinase-like substance in the human plasma hydrolyzed fibrinogen, gelatin and α-casein, but did not the serum albumin, the hemoglobin and γ-globulin. The fibrinogenolytic activity in the human plasma was detected in all of six healthy subjects. The activity was disappeared at 50℃ for 30min, and inhibited by 10^<-3>M DFP or anti-human plasminogen IgG. The proteinase-like substance was bound on lysine-Sepharose CL-4B and eluted with 0.2M ε-aminocaprylic acid. The examination by gel filtration induced that the fibrinolytic activity existed in the fraction corresponding to the molecular weight of 90kDa. By the immunoblotting method, a mobility of the substance was shown to be similar to that of human plasminogen. The data demonstrated that the plasmin-like proteinase activity in the human plasma was artifact something made from plasminogen. | |||||||||||
| 書誌情報 |
武庫川女子大学紀要. 自然科学編 巻 45, p. 107-111, 発行日 1998-03-31 |
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| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 0916-3123 | |||||||||||
| 書誌レコードID | ||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||
| 収録物識別子 | AN10187277 | |||||||||||
