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  1. 武庫川女子大学紀要 自然科学編
  2. 45巻(1997年)

基質を含むポリアクリルアミド・ゲル電気泳動法によるヒト血漿中の中性セリンプロテイナーゼの検出の試み

https://doi.org/10.14993/00000332
https://doi.org/10.14993/00000332
79b8c76a-c03e-435b-8e03-29ffb6afbae9
名前 / ファイル ライセンス アクション
P107-111.pdf P107-111.pdf (645.1 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2009-06-11
タイトル
タイトル 基質を含むポリアクリルアミド・ゲル電気泳動法によるヒト血漿中の中性セリンプロテイナーゼの検出の試み
タイトル
言語 en
タイトル Studies on detection of neutral serine proteinase in human plasma by electrophoresis using substrate-containing polyacrylamide gel
言語
言語 jpn
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ departmental bulletin paper
ID登録
ID登録 10.14993/00000332
ID登録タイプ JaLC
著者 堀江, 登

× 堀江, 登

WEKO 1301

堀江, 登

ja-Kana ホリエ, ノボル

en HORIE, Noboru

Search repository
抄録
内容記述タイプ Abstract
内容記述 In order to examine the existence of proteinase in the human plasma, the electrophoretic method by using polyacrylamide-gel containing SDS and substrate was adopted in this study. The proteinase-like substance in the human plasma hydrolyzed fibrinogen, gelatin and α-casein, but did not the serum albumin, the hemoglobin and γ-globulin. The fibrinogenolytic activity in the human plasma was detected in all of six healthy subjects. The activity was disappeared at 50℃ for 30min, and inhibited by 10^<-3>M DFP or anti-human plasminogen IgG. The proteinase-like substance was bound on lysine-Sepharose CL-4B and eluted with 0.2M ε-aminocaprylic acid. The examination by gel filtration induced that the fibrinolytic activity existed in the fraction corresponding to the molecular weight of 90kDa. By the immunoblotting method, a mobility of the substance was shown to be similar to that of human plasminogen. The data demonstrated that the plasmin-like proteinase activity in the human plasma was artifact something made from plasminogen.
書誌情報 武庫川女子大学紀要. 自然科学編

巻 45, p. 107-111, 発行日 1998-03-31
ISSN
収録物識別子タイプ ISSN
収録物識別子 0916-3123
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AN10187277
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