WEKO3
アイテム
基質を含むポリアクリルアミド・ゲル電気泳動法によるヒト血漿中の中性セリンプロテイナーゼの検出の試み
https://doi.org/10.14993/00000332
https://doi.org/10.14993/0000033279b8c76a-c03e-435b-8e03-29ffb6afbae9
名前 / ファイル | ライセンス | アクション |
---|---|---|
![]() |
|
Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
公開日 | 2009-06-11 | |||||||||||
タイトル | ||||||||||||
タイトル | 基質を含むポリアクリルアミド・ゲル電気泳動法によるヒト血漿中の中性セリンプロテイナーゼの検出の試み | |||||||||||
タイトル | ||||||||||||
タイトル | Studies on detection of neutral serine proteinase in human plasma by electrophoresis using substrate-containing polyacrylamide gel | |||||||||||
言語 | en | |||||||||||
言語 | ||||||||||||
言語 | jpn | |||||||||||
資源タイプ | ||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
資源タイプ | departmental bulletin paper | |||||||||||
ID登録 | ||||||||||||
ID登録 | 10.14993/00000332 | |||||||||||
ID登録タイプ | JaLC | |||||||||||
著者 |
堀江, 登
× 堀江, 登
|
|||||||||||
抄録 | ||||||||||||
内容記述タイプ | Abstract | |||||||||||
内容記述 | In order to examine the existence of proteinase in the human plasma, the electrophoretic method by using polyacrylamide-gel containing SDS and substrate was adopted in this study. The proteinase-like substance in the human plasma hydrolyzed fibrinogen, gelatin and α-casein, but did not the serum albumin, the hemoglobin and γ-globulin. The fibrinogenolytic activity in the human plasma was detected in all of six healthy subjects. The activity was disappeared at 50℃ for 30min, and inhibited by 10^<-3>M DFP or anti-human plasminogen IgG. The proteinase-like substance was bound on lysine-Sepharose CL-4B and eluted with 0.2M ε-aminocaprylic acid. The examination by gel filtration induced that the fibrinolytic activity existed in the fraction corresponding to the molecular weight of 90kDa. By the immunoblotting method, a mobility of the substance was shown to be similar to that of human plasminogen. The data demonstrated that the plasmin-like proteinase activity in the human plasma was artifact something made from plasminogen. | |||||||||||
書誌情報 |
武庫川女子大学紀要. 自然科学編 巻 45, p. 107-111, 発行日 1998-03-31 |
|||||||||||
ISSN | ||||||||||||
収録物識別子タイプ | ISSN | |||||||||||
収録物識別子 | 0916-3123 | |||||||||||
書誌レコードID | ||||||||||||
収録物識別子タイプ | NCID | |||||||||||
収録物識別子 | AN10187277 |