{"created":"2023-05-15T12:05:52.862183+00:00","id":338,"links":{},"metadata":{"_buckets":{"deposit":"c574cb3b-abc4-475b-9e82-1eaad6b5691c"},"_deposit":{"created_by":3,"id":"338","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"338"},"status":"published"},"_oai":{"id":"oai:mukogawa.repo.nii.ac.jp:00000338","sets":["267:269"]},"author_link":["1301"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1998-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"111","bibliographicPageStart":"107","bibliographicVolumeNumber":"45","bibliographic_titles":[{"bibliographic_title":"武庫川女子大学紀要. 自然科学編"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In order to examine the existence of proteinase in the human plasma, the electrophoretic method by using polyacrylamide-gel containing SDS and substrate was adopted in this study. The proteinase-like substance in the human plasma hydrolyzed fibrinogen, gelatin and α-casein, but did not the serum albumin, the hemoglobin and γ-globulin. The fibrinogenolytic activity in the human plasma was detected in all of six healthy subjects. The activity was disappeared at 50℃ for 30min, and inhibited by 10^<-3>M DFP or anti-human plasminogen IgG. The proteinase-like substance was bound on lysine-Sepharose CL-4B and eluted with 0.2M ε-aminocaprylic acid. The examination by gel filtration induced that the fibrinolytic activity existed in the fraction corresponding to the molecular weight of 90kDa. By the immunoblotting method, a mobility of the substance was shown to be similar to that of human plasminogen. The data demonstrated that the plasmin-like proteinase activity in the human plasma was artifact something made from plasminogen.","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.14993/00000332","subitem_identifier_reg_type":"JaLC"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10187277","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0916-3123","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"堀江, 登"},{"creatorName":"ホリエ, ノボル","creatorNameLang":"ja-Kana"},{"creatorName":"HORIE, Noboru","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-09-25"}],"displaytype":"detail","filename":"P107-111.pdf","filesize":[{"value":"645.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"P107-111.pdf","url":"https://mukogawa.repo.nii.ac.jp/record/338/files/P107-111.pdf"},"version_id":"466aa290-90b3-475f-beb7-dac3a10a7f61"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"基質を含むポリアクリルアミド・ゲル電気泳動法によるヒト血漿中の中性セリンプロテイナーゼの検出の試み","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"基質を含むポリアクリルアミド・ゲル電気泳動法によるヒト血漿中の中性セリンプロテイナーゼの検出の試み"},{"subitem_title":"Studies on detection of neutral serine proteinase in human plasma by electrophoresis using substrate-containing polyacrylamide gel","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"3","path":["269"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-06-11"},"publish_date":"2009-06-11","publish_status":"0","recid":"338","relation_version_is_last":true,"title":["基質を含むポリアクリルアミド・ゲル電気泳動法によるヒト血漿中の中性セリンプロテイナーゼの検出の試み"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-05-15T13:31:36.225525+00:00"}