{"_buckets": {"deposit": "00460c36-d1d2-45f4-bd43-5cadea908a4f"}, "_deposit": {"created_by": 3, "id": "348", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "348"}, "status": "published"}, "_oai": {"id": "oai:mukogawa.repo.nii.ac.jp:00000348", "sets": ["270"]}, "author_link": ["1301", "6149", "6145"], "item_10002_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1999-03-31", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "50", "bibliographicPageStart": "47", "bibliographicVolumeNumber": "46", "bibliographic_titles": [{"bibliographic_title": "武庫川女子大学紀要. 自然科学編"}]}]}, "item_10002_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "An improved method to determine the molecular weight of the subunit of lactate dehydrogenase and malate dehydrogenase after sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) has been developed. This method was based on the finding that on a gel, which was washed with a buffer to remove SDS after SDS-PAGE, stained with an enzymatic activity staining mixture and then stained with coomassie blue, there appeared one active stained band with apparent molecular weights of 35,500 (monomer) (lactate dehydrogenase) and 31,000 (monomer) (malate dehydrogenase) on the SDS-PAGE gel. The method developed here may be applicable to a wide range of active stainable-enzymes as a rapid and simple molecular weight determination method of the subunit after SDS-PAGE.", "subitem_description_type": "Abstract"}]}, "item_10002_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.14993/00000342", "subitem_identifier_reg_type": "JaLC"}]}, "item_10002_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "武庫川女子大学"}]}, "item_10002_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN10187277", "subitem_source_identifier_type": "NCID"}]}, "item_10002_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0916-3123", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "岡村, 徳光"}, {"creatorName": "オカムラ, トクミツ", "creatorNameLang": "ja-Kana"}, {"creatorName": "OKAMURA, Tokumitsu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6145", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "堀江, 登"}, {"creatorName": "ホリエ, ノボル", "creatorNameLang": "ja-Kana"}, {"creatorName": "HORIE, Noboru", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "1301", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "大杉, 匡弘"}, {"creatorName": "オオスギ, マサヒロ", "creatorNameLang": "ja-Kana"}, {"creatorName": "OHSUGI, Masahiro ", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6149", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-09-25"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "P047-050.pdf", "filesize": [{"value": "324.8 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 324800.0, "url": {"label": "P047-050.pdf", "url": "https://mukogawa.repo.nii.ac.jp/record/348/files/P047-050.pdf"}, "version_id": "ec9d18fc-6dba-48b5-949b-e37aa9dee14b"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "An Improved Convenient Molecular Weight-determination Method of Subunit for Active Stainable-Enzyme after SDS Electrophoresis", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "An Improved Convenient Molecular Weight-determination Method of Subunit for Active Stainable-Enzyme after SDS Electrophoresis", "subitem_title_language": "en"}]}, "item_type_id": "10002", "owner": "3", "path": ["270"], "permalink_uri": "https://doi.org/10.14993/00000342", "pubdate": {"attribute_name": "公開日", "attribute_value": "2009-06-30"}, "publish_date": "2009-06-30", "publish_status": "0", "recid": "348", "relation": {}, "relation_version_is_last": true, "title": ["An Improved Convenient Molecular Weight-determination Method of Subunit for Active Stainable-Enzyme after SDS Electrophoresis"], "weko_shared_id": 3}