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An Improved Convenient Molecular Weight-determination Method of Subunit for Active Stainable-Enzyme after SDS Electrophoresis
https://doi.org/10.14993/00000342
https://doi.org/10.14993/0000034227f7f3f2-3634-4b80-a0da-67ad46f8790a
| 名前 / ファイル | ライセンス | アクション |
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P047-050.pdf (324.8 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||||||||||||||
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| 公開日 | 2009-06-30 | |||||||||||||||||||||||||
| タイトル | ||||||||||||||||||||||||||
| タイトル | An Improved Convenient Molecular Weight-determination Method of Subunit for Active Stainable-Enzyme after SDS Electrophoresis | |||||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||||||
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| 言語 | eng | |||||||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||||||||||||||||
| ID登録 | ||||||||||||||||||||||||||
| ID登録 | 10.14993/00000342 | |||||||||||||||||||||||||
| ID登録タイプ | JaLC | |||||||||||||||||||||||||
| 著者 |
岡村, 徳光
× 岡村, 徳光
× 堀江, 登
× 大杉, 匡弘
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| 抄録 | ||||||||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||||||||
| 内容記述 | An improved method to determine the molecular weight of the subunit of lactate dehydrogenase and malate dehydrogenase after sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) has been developed. This method was based on the finding that on a gel, which was washed with a buffer to remove SDS after SDS-PAGE, stained with an enzymatic activity staining mixture and then stained with coomassie blue, there appeared one active stained band with apparent molecular weights of 35,500 (monomer) (lactate dehydrogenase) and 31,000 (monomer) (malate dehydrogenase) on the SDS-PAGE gel. The method developed here may be applicable to a wide range of active stainable-enzymes as a rapid and simple molecular weight determination method of the subunit after SDS-PAGE. | |||||||||||||||||||||||||
| 書誌情報 |
武庫川女子大学紀要. 自然科学編 巻 46, p. 47-50, 発行日 1999-03-31 |
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| 出版者 | ||||||||||||||||||||||||||
| 出版者 | 武庫川女子大学 | |||||||||||||||||||||||||
| ISSN | ||||||||||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||||||||||
| 収録物識別子 | 0916-3123 | |||||||||||||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||||||||||||
| 収録物識別子 | AN10187277 | |||||||||||||||||||||||||
