{"_buckets": {"deposit": "e2aaa8e5-67b1-4d37-9b02-8a182c3acc52"}, "_deposit": {"created_by": 3, "id": "343", "owners": [3], "pid": {"revision_id": 0, "type": "depid", "value": "343"}, "status": "published"}, "_oai": {"id": "oai:mukogawa.repo.nii.ac.jp:00000343", "sets": ["270"]}, "author_link": ["6221", "6370", "6369", "6373"], "item_10002_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1999-03-31", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "26", "bibliographicPageStart": "21", "bibliographicVolumeNumber": "46", "bibliographic_titles": [{"bibliographic_title": "武庫川女子大学紀要. 自然科学編"}]}]}, "item_10002_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Two low molecular weight proteins were separately isolated in crystalline forms from buckwheat seeds by affinity chromatography on a chitin column, ion exchange chromatography on a CM-cellulose column and gel filtration through a Sephadex G-25 column. The two crystalline proteins thus obtained were referred to as buckwheat chitin-binding proteins I and II, BCP I and II, respectively. Further purification of the crystalline BCP II by high performance liquid chromatography resulted in separation into the three proteins, BCP IIa1, IIa2 and IIb, respectively. These three purified proteins showed a single band on SDS-polyacrylamide gel electrophoresis, indicating that each protein was essentially homogeneous. The purified BCP IIa1, IIa2 and IIb had the very similar amino acid compositions, mutually, and comprised the total numbers of amino acid residues of 40 to 46, suggesting that they are closely related to each other. The amino acid compositions of the purified proteins revealed that the minimum molecular weights of BCP IIa1, IIa2 and IIb were 4,800, 5,500 and 4,800, respectively. The proteins were stable in the wide range of pH3 to 11 at lower temperature than 60℃ and even in the range of acidic pH at 100℃.", "subitem_description_type": "Abstract"}]}, "item_10002_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.14993/00000337", "subitem_identifier_reg_type": "JaLC"}]}, "item_10002_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN10187277", "subitem_source_identifier_type": "NCID"}]}, "item_10002_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0916-3123", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "坂本, えり奈"}, {"creatorName": "サカモト, エリナ", "creatorNameLang": "ja-Kana"}, {"creatorName": "SAKAMOTO, Erina", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6369", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "家本, 敦子"}], "nameIdentifiers": [{"nameIdentifier": "6370", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "清原, 利文"}, {"creatorName": "キヨハラ, トシブミ", "creatorNameLang": "ja-Kana"}, {"creatorName": "KIYOHARA, Toshibumi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6221", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Iemoto, Atsuko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "6373", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2017-09-25"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "P021-026.pdf", "filesize": [{"value": "739.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 739600.0, "url": {"label": "P021-026.pdf", "url": "https://mukogawa.repo.nii.ac.jp/record/343/files/P021-026.pdf"}, "version_id": "06c8ce02-72f5-42bf-abb6-f4c3a22cabae"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "ソバ種実から得られる低分子性タンパク質の精製と2,3の理化学的性質", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "ソバ種実から得られる低分子性タンパク質の精製と2,3の理化学的性質"}, {"subitem_title": "Purification and Physicochemical Properties of Low Molecular Weight Proteins from Buckwheat Seeds", "subitem_title_language": "en"}]}, "item_type_id": "10002", "owner": "3", "path": ["270"], "permalink_uri": "https://doi.org/10.14993/00000337", "pubdate": {"attribute_name": "公開日", "attribute_value": "2009-06-15"}, "publish_date": "2009-06-15", "publish_status": "0", "recid": "343", "relation": {}, "relation_version_is_last": true, "title": ["ソバ種実から得られる低分子性タンパク質の精製と2,3の理化学的性質"], "weko_shared_id": 3}