WEKO3
アイテム
Isolation and Culture of Protoplasts from Gymnocalycium baldianum (speg.) Speg. (Hikagyoku) Callus Cultures
https://doi.org/10.14993/00000323
https://doi.org/10.14993/0000032374f3c96e-34ed-45a5-b7de-a06b4a7618b2
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
P053-055.pdf (731.9 kB)
|
|
| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2009-06-10 | |||||||||||||||||
| タイトル | ||||||||||||||||||
| タイトル | Isolation and Culture of Protoplasts from Gymnocalycium baldianum (speg.) Speg. (Hikagyoku) Callus Cultures | |||||||||||||||||
| 言語 | en | |||||||||||||||||
| 言語 | ||||||||||||||||||
| 言語 | eng | |||||||||||||||||
| 資源タイプ | ||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||||||||
| ID登録 | ||||||||||||||||||
| ID登録 | 10.14993/00000323 | |||||||||||||||||
| ID登録タイプ | JaLC | |||||||||||||||||
| 著者 |
岡村, 徳光
× 岡村, 徳光
× 松尾, 志保
× MATSUO, Shiho
|
|||||||||||||||||
| 抄録 | ||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||
| 内容記述 | Isolation and culture of protoplasts from Gymnocalycium baldianum (Hikagyoku) callus cultures were examined. The peeling treatment of fruit largely excluded contamination during the induction and culture of callus, leading to successful formation of callus. Callus was induced effectively from the flesh using MS solid medium containing 0.01μM of kinetin and 0.01μM of 2, 4-dichlorophenoxyacetic acid (2, 4-D). The callus growth in the medium was largely promoted without causing a browning of the callus in the subculture. In preparation of protoplasts, the enzyme solution gave a high yield of protoplasts. Cell division into 2 cells was observed after 24 hours of culture, then micro colonies formed after 7 days, followed by colony formation within 2 weeks. | |||||||||||||||||
| 書誌情報 |
武庫川女子大学紀要. 自然科学編 巻 45, p. 53-55, 発行日 1998-03-31 |
|||||||||||||||||
| 出版者 | ||||||||||||||||||
| 出版者 | 武庫川女子大学 | |||||||||||||||||
| ISSN | ||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||
| 収録物識別子 | 0916-3123 | |||||||||||||||||
| 書誌レコードID | ||||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||||
| 収録物識別子 | AN10187277 | |||||||||||||||||
