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サイトメガロウイルス感染症の迅速DNA診断法の開発
https://doi.org/10.14993/00000330
https://doi.org/10.14993/0000033046411cd5-505c-4a9f-81dd-573c89112860
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
P095-100.pdf (612.2 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||
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| 公開日 | 2009-06-11 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | サイトメガロウイルス感染症の迅速DNA診断法の開発 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Establishment of rapid diagnosis technique of Cytomegalovirus infection using polymerase chain reaction | |||||||||||||
| 言語 | en | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | jpn | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||||
| ID登録 | ||||||||||||||
| ID登録 | 10.14993/00000330 | |||||||||||||
| ID登録タイプ | JaLC | |||||||||||||
| 著者 |
中村, 一江
× 中村, 一江
× 家本, 敦子
× Nakamura, Kazue
× Iemoto, Atsuko
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | The conventional diagnostic procedures for CMV infections have been both isolation of viruses and titeration of serum antibodies. These methods need several days for the final diagnosis, causing a delay in the diagnosis. Therefore, a rapid diagnostic procedure has been highly required. In this study, we conducted polymerase chain reaction (PCR) using three sets of primers corresponding IE-, LA- and V-regions of the CMV genome. Using the former two sets of primers, many bands were amplified from a DNA sample isolated from the peripheral blood leukocytes of a patient with CMV infection. However, using the latter set of primers (V-region), a single band of 305bp was amplified. Using these V-region primers, no hybridization was needed to detect the specific band. No PCR amplification products was detected by using the V-region primers from DNAs isolated from normal subjects. We also tried to use urine sedimentation samples without extraction of DNA. Sedimented cells were resuspended in water, heated by a microwave oven and used for PCR templates. A 305bp band was also detected. Subsequently, the entire procedure of our method to detect the CMV DNA requires only 4 hours. Therefore, this PCR method is reliable and useful in making rapid diagnosis of CMV infection. | |||||||||||||
| 書誌情報 |
武庫川女子大学紀要. 自然科学編 巻 45, p. 95-100, 発行日 1998-03-31 |
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| ISSN | ||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 0916-3123 | |||||||||||||
| 書誌レコードID | ||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||
| 収録物識別子 | AN10187277 | |||||||||||||
