{"created":"2023-05-15T12:05:52.775783+00:00","id":336,"links":{},"metadata":{"_buckets":{"deposit":"3bd6942a-0d9e-4821-9872-0505d6696c95"},"_deposit":{"created_by":3,"id":"336","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"336"},"status":"published"},"_oai":{"id":"oai:mukogawa.repo.nii.ac.jp:00000336","sets":["267:269"]},"author_link":["6339","6340","6337","6338"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1998-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"100","bibliographicPageStart":"95","bibliographicVolumeNumber":"45","bibliographic_titles":[{"bibliographic_title":"武庫川女子大学紀要. 自然科学編"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The conventional diagnostic procedures for CMV infections have been both isolation of viruses and titeration of serum antibodies. These methods need several days for the final diagnosis, causing a delay in the diagnosis. Therefore, a rapid diagnostic procedure has been highly required. In this study, we conducted polymerase chain reaction (PCR) using three sets of primers corresponding IE-, LA- and V-regions of the CMV genome. Using the former two sets of primers, many bands were amplified from a DNA sample isolated from the peripheral blood leukocytes of a patient with CMV infection. However, using the latter set of primers (V-region), a single band of 305bp was amplified. Using these V-region primers, no hybridization was needed to detect the specific band. No PCR amplification products was detected by using the V-region primers from DNAs isolated from normal subjects. We also tried to use urine sedimentation samples without extraction of DNA. Sedimented cells were resuspended in water, heated by a microwave oven and used for PCR templates. A 305bp band was also detected. Subsequently, the entire procedure of our method to detect the CMV DNA requires only 4 hours. Therefore, this PCR method is reliable and useful in making rapid diagnosis of CMV infection.","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.14993/00000330","subitem_identifier_reg_type":"JaLC"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10187277","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0916-3123","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"中村, 一江"}],"nameIdentifiers":[{"nameIdentifier":"6337","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"家本, 敦子"}],"nameIdentifiers":[{"nameIdentifier":"6338","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakamura, Kazue","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6339","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Iemoto, Atsuko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6340","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-09-25"}],"displaytype":"detail","filename":"P095-100.pdf","filesize":[{"value":"612.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"P095-100.pdf","url":"https://mukogawa.repo.nii.ac.jp/record/336/files/P095-100.pdf"},"version_id":"3914f98b-e33f-40c6-a93c-6329ef635a7a"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"サイトメガロウイルス感染症の迅速DNA診断法の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"サイトメガロウイルス感染症の迅速DNA診断法の開発"},{"subitem_title":"Establishment of rapid diagnosis technique of Cytomegalovirus infection using polymerase chain reaction","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"3","path":["269"],"pubdate":{"attribute_name":"公開日","attribute_value":"2009-06-11"},"publish_date":"2009-06-11","publish_status":"0","recid":"336","relation_version_is_last":true,"title":["サイトメガロウイルス感染症の迅速DNA診断法の開発"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-05-16T10:41:27.601478+00:00"}