| アイテムタイプ |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2013-05-17 |
| タイトル |
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|
タイトル |
Tendergreen と遺伝子組換えエンドウ由来α－アミラーゼインヒビターの糖鎖構造 |
| タイトル |
|
|
タイトル |
Structures of sugar chains in α-amylase inhibitors from Tendergreen（Phaseolus vulgaris L.） and transgenic field pea（Pisum sativum L.） |
|
言語 |
en |
| 言語 |
|
|
言語 |
jpn |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
departmental bulletin paper |
| ID登録 |
|
|
ID登録 |
10.14993/00000517 |
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ID登録タイプ |
JaLC |
| 著者 |
澤田, 小百合
田代, 操
Tashiro, Misao
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| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
We isolated an α-amylase inhibitor（ AI） from transgenic field pea in which a Tendergreen α-amylase inhibitor gene was transduced and the sugar chain structures of AIs from transgenic field pea and Tendergreen bean were compared. Plural electrophoretic bands were found in each AI and each subunit of each AI and these electrophoretic patterns were different between transgenic field pea and Tendergreen. However, each subunit with the deglycosylation using TFMSA showed single band. The structure of a major sugar chain of α subunit of Tendergreen AI was M9. Structures of major sugar chains of α subunit of transgenic field pea AI were M8 and M9. On the other hand, major sugar chains in the βsubunits were the same, but the compositions were slightly different. Transgenic field pea AI and Tendergreen AI have heterogeneity in the composition of the binding sugar chains. |
| 書誌情報 |
武庫川女子大学紀要. 自然科学編
巻 60,
p. 29-33,
発行日 2013-03-31
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| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0916-3123 |
| 書誌レコードID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN10187277 |
P29-33.pdf (8.1 MB)
