| アイテムタイプ |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2009-07-01 |
| タイトル |
|
|
タイトル |
脂質過酸化物により劣化させられたチューブリンのGTPase活性に対するSH基還元剤(グルタチオン・システイン)の回復効果 |
| タイトル |
|
|
タイトル |
The Protective Effect of SH Reductants (Glutathione and Cysteine) on Tubulin GTPase Activity Deteriorated by Lipid Peroxides |
|
言語 |
en |
| 言語 |
|
|
言語 |
jpn |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| ID登録 |
|
|
ID登録 |
10.14993/00000363 |
|
ID登録タイプ |
JaLC |
| 著者 |
川上, 美佐子
中野, 美江
清原, 利文
土井, 裕司
Kawakami, Misako
Nakano, Mie
Doi, Hiroshi
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Tubulin, which is a main component of the cytoskeleton, plays various important roles in cellular processes such as axonal transport, ciliary and flagellar movement, cell division, and cell segregation in the form of microtubules. The aim of the present study was to examine the effect of SH reductants on phospholipid peroxides-induced inhibition of tubulin GTPase activity. Lipid peroxides were prepared by photooxidation of lipoid S100 of phospholipid obtained from soybean. Tubulin GTPase activity was most inhibited by lipid photooxidized for 24 hours among ones for 16 and 24 hours. The more the amount of lipid peroxides is added, the more the activity is inhibited. Tubulin GTPase activity was lowered even in the presence of a slight amount of lipid peroxides. This suggests that the presence of a slight amount of lipid peroxides influences cellular functions in vivo. Prepared lipid peroxides was identified as a monohydroperoxide by an electrochemical detection through HPLC and a mass spectrometry. Glutathione and cysteine was used as water soluble reductants. Tubulin GTPase activity deteriorated by lipid peroxides was restored by the addition of water soluble reductants. The results presented above suggest that glutathione and cysteine have a protective effect on cellular aging by the reduction of materials oxidized in vivo. |
| 書誌情報 |
武庫川女子大学紀要. 自然科学編
巻 47,
p. 29-34,
発行日 2000-03-31
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| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0916-3123 |
| 書誌レコードID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN10187277 |
P029-034.pdf (498.8 kB)
