{"created":"2024-06-27T07:07:36.008587+00:00","id":2000295,"links":{},"metadata":{"_buckets":{"deposit":"07c2b10d-080a-4a17-ad7b-ae3759090e35"},"_deposit":{"created_by":16,"id":"2000295","owner":"16","owners":[16],"pid":{"revision_id":0,"type":"depid","value":"2000295"},"status":"published"},"_oai":{"id":"oai:mukogawa.repo.nii.ac.jp:02000295","sets":["535","535:1719459620857"]},"author_link":["9383","9645","10029","10030","10031","2491"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2022-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"22","bibliographicPageStart":"17","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"栄養科学研究","bibliographic_titleLang":"ja"},{"bibliographic_title":"The Mukogawa journal of nutrition science research","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"酵母の有するエチルアルコール脱水素酵素（ADH）は、エチルアルコール酸化およびアセトアルデヒド還元の両反応に関与し、分子量は150 kDaであることが報告されている。これまでに、担子菌はエチルアルコールを生成することが明らかにされているが、アセトアルデヒド還元反応に関与するADHの存在については報告されていない。本研究では、アセトアルデヒド還元活性を有するADHの存在を明らかにし、ワインの生産を試みた。エノキタケの粗酵素液においてNative-PAGE後のADH活性染色の結果、ポリアクリルアミドゲル上にてアセトアルデヒド還元活性を有するADHおよびエチルアルコール脱水素活性を有するADHの染色バンドが確認された。今回検討した糖濃度、発酵期間において最も高いアルコール生成が認められたのは、糖度15％（w/v）、発酵期間５週間であった。菌糸体重量とアルコール濃度の関係からアルコールの生成には、一定量の菌糸が必要であることが示唆された。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"The production of ethyl alcohol by mushrooms has been elucidated. However, the existence of alcohol dehydrogenase（ ADH） that produces ethyl alcohol remains unclear. The purpose of this study was to clarify the presence of ADH that produces ethyl alcohol or ADH that decomposes ethyl alcohol in mushrooms. Furthermore, we tried to prepare wine using ADH of mushrooms. As a result of ADH activity staining after native-PAGE in the crude enzyme solution of mushroom, the staining bands of ADH that decomposes ethyl alcohol and ADH that produces ethyl alcohol were confirmed on the polyacrylamide gel. In wine of mushroom, the highest alcohol production was observed with a sugar content of 15% and a fermentation period of 5 weeks wonder conditions in this study. The relationship between mycelial weight and alcohol concentration suggested that a certain amount of mycelia was required for alcohol production.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.14993/0002000295","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"武庫川学院","subitem_publisher_language":"ja"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12744102","subitem_source_identifier_type":"NCID"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open 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Naomi","creatorNameLang":"en"}],"familyNames":[{"familyName":"竹本","familyNameLang":"ja"},{"familyName":"タケモト","familyNameLang":"ja-Kana"},{"familyName":"TAKEMOTO","familyNameLang":"en"}],"givenNames":[{"givenName":"尚未","givenNameLang":"ja"},{"givenName":"ナオミ","givenNameLang":"ja-Kana"},{"givenName":"Naomi","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"9645","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"佐々木, 裕子","creatorNameLang":"ja"},{"creatorName":"ササキ, ヒロコ","creatorNameLang":"ja-Kana"},{"creatorName":"SASAKI, Hiroko","creatorNameLang":"en"}],"familyNames":[{"familyName":"佐々木","familyNameLang":"ja"},{"familyName":"ササキ","familyNameLang":"ja-Kana"},{"familyName":"SASAKI","familyNameLang":"en"}],"givenNames":[{"givenName":"裕子","givenNameLang":"ja"},{"givenName":"ヒロコ","givenNameLang":"ja-Kana"},{"givenName":"Hiroko","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"10029","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"小森, 有紀","creatorNameLang":"ja"},{"creatorName":"コモリ, ユキ","creatorNameLang":"ja-Kana"},{"creatorName":"KOMORI, Yuki","creatorNameLang":"en"}],"familyNames":[{"familyName":"小森","familyNameLang":"ja"},{"familyName":"コモリ","familyNameLang":"ja-Kana"},{"familyName":"KOMORI","familyNameLang":"en"}],"givenNames":[{"givenName":"有紀","givenNameLang":"ja"},{"givenName":"ユキ","givenNameLang":"ja-Kana"},{"givenName":"Yuki","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"10030","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"山口, 真弥","creatorNameLang":"ja"},{"creatorName":"ヤマグチ, マヤ","creatorNameLang":"ja-Kana"},{"creatorName":"YAMAGUCHI, Maya","creatorNameLang":"en"}],"familyNames":[{"familyName":"山口","familyNameLang":"ja"},{"familyName":"ヤマグチ","familyNameLang":"ja-Kana"},{"familyName":"YAMAGUCHI","familyNameLang":"en"}],"givenNames":[{"givenName":"真弥","givenNameLang":"ja"},{"givenName":"マヤ","givenNameLang":"ja-Kana"},{"givenName":"Maya","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"10031","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"松井, 徳光","creatorNameLang":"ja"},{"creatorName":"マツイ, トクミツ","creatorNameLang":"ja-Kana"},{"creatorName":"MATSUI, Tokumitsu","creatorNameLang":"en"}],"familyNames":[{"familyName":"松井","familyNameLang":"ja"},{"familyName":"マツイ","familyNameLang":"ja-Kana"},{"familyName":"MATSUI","familyNameLang":"en"}],"givenNames":[{"givenName":"徳光","givenNameLang":"ja"},{"givenName":"トクミツ","givenNameLang":"ja-Kana"},{"givenName":"Tokumitsu","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2491","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-06-27"}],"displaytype":"detail","filename":"P17-22.pdf","filesize":[{"value":"945 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