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Calcium binding to the tubulin-colchicine complex in the state of GTP in a BES buffer
https://doi.org/10.14993/00000420
https://doi.org/10.14993/000004200c7fd689-905e-497a-a4fa-8682a84f7b79
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
P067-072.pdf (517.4 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||||
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| 公開日 | 2009-07-20 | |||||||||||||||
| タイトル | ||||||||||||||||
| タイトル | Calcium binding to the tubulin-colchicine complex in the state of GTP in a BES buffer | |||||||||||||||
| 言語 | en | |||||||||||||||
| 言語 | ||||||||||||||||
| 言語 | eng | |||||||||||||||
| 資源タイプ | ||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||||||
| ID登録 | ||||||||||||||||
| ID登録 | 10.14993/00000420 | |||||||||||||||
| ID登録タイプ | JaLC | |||||||||||||||
| 著者 |
土井, 裕司
× 土井, 裕司
× 川口, 真規子
× Doi, Hiroshi
× Kawaguchi, Makiko
× Timasheff, Serge N.
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| 抄録 | ||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||
| 内容記述 | Magnesium and calcium binding were assayed using the tubulin-colchicine complex in a BES buffer, in which the calcium binding to tubulin had been measured by Grisham et al. (Fed. Proc. 39, 2162). In the previous paper, an imidazole buffer was used as the buffer which does not chelate to calcium and which is substituted to phosphate buffer. The result of calcium binding measurement indicated the same binding constant between at pH 7.0 and at pH 6.5 in the absence of magnesium (1.08×10^<-5> M at pH 7.0 and 1.10×10^<-5> M at pH 6.5). Also, the calcium binding constant of the tubulin-colchicine complex was the same as that of tubulin in a BES buffer, pH 7.0. The increase of magnesium concentration inhibited calcium binding to the tubulin-colchicine complex. The affinity of calcium at pH 7.0 was lower than that at pH 6.5 in the presence of magnesium. This leads the effect of calcium on the polymerization of the tubulin-colchicine complex is small at pH 6.5 rather than at pH 7.0. The results obtained in the polymerization experiment were in good agreement with the results of the calcium binding experiment. | |||||||||||||||
| 書誌情報 |
武庫川女子大学紀要. 自然科学編 巻 50, p. 67-72, 発行日 2003-03-31 |
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| ISSN | ||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||
| 収録物識別子 | 0916-3123 | |||||||||||||||
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| 収録物識別子タイプ | NCID | |||||||||||||||
| 収録物識別子 | AN10187277 | |||||||||||||||
