We report the effect of culture broths from various microorganisms on fruiting of Pleurotus ostreatus W0001. P. ostreatus W0001 has been grown on sterile sawdust medium containing culture broths from various microorganisms with good yields in small scale experiments. The spawning to first yield obtained for a period of 10 days was 9.7% of the moistened medium on the sawdusts medium containing culture broth from Saccharomyces cerevisiae AKU 4100.