A mixture of callus of Akamatsu and mycelium of Tricholoma matsutake was obtained from roots of it with MS medium containing 0.01μM of 2, 4-D and 0.1μM of kinetin. Colony, which formed polymer of mycelia of T. matsutake, was obtained with MS medium containing 0.01μM of IAA and 0.01μM of kinetin. Therefore, it may be possible to cultivate T. matsutake by means of using hormones such as IAA and kinetin on the sawdust medium.