Isolation and fusion of protoplasts from Basella rubra leaf and stem were examined. In preparation of protoplasts, the enzyme solution gave a high yield of protoplasts. The diameter of protoplasts induced ranged from 30μm to 120μm. Cell division into 2 cells was observed after 24 hours of culture, then micro colonies formed after 7 days, followed by colony formation within 2 weeks
Isolation and Fusion of Protoplasts from Basella rubra Leaf and Stem Cultures
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