The fusion method using PEG as a fusogenic agent has a number of disadvantages in comparison with the newly developed electrical fusion method. However, there has been little application yet of this electrical fusion method to the fusion of protoplasts of mushrooms. In this report, we describe the pearl chain formation and effect of pulse field strength on protoplast breakdown of protoplasts prepared from Hericium erinaceum. The protoplasts density favorable for pearl chain formation was about 10^8protoplasts/ml, and the yield of single pairs was maximum (30%) after 60sec dielectrophoresis at 100V/cm and 1MHz. The field intensity required to decompose half of the protoplast from H. erinaceum was 8kV/cm in a 0.7M mannitol solution.
Dielectrophoresis Conditions for Pearl Chain Formation and Effect of Pulse Field Strength on Protoplast Breakdown of Hericium erinaceum
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